trim33 gfp Search Results


85
Addgene inc trim33 gfp
Trim33 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trim33+gfp/TRIM33-GFP+(Plasmid+%2365399)/pmc04666613-221-0-7
Average 85 stars, based on 1 article reviews
trim33 gfp - by Bioz Stars, 2026-10
85/100 stars
  Buy from Supplier

90
Addgene inc trim33 plasmid
FIGURE 2. KAP1 inhibits RNA viralinduced IFN-b activation. (A) Luciferase analysis of IFN-b reporter activation in HEK293T cells trans- fected with IFN-b reporter plasmid with empty control, TRIM28 (KAP1), TRIM24, <t>TRIM33,</t> or TRIM66 expression plasmid and then infected with SeV for 12 h. (B) Luciferase analysis of IFN-b reporter activation in HEK293T cells transfected with IFN-b reporter plasmid, together with empty control plasmid or KAP1 expression plasmids, and then infected with VSV for 12 h. (C) Western blot analysis of KAP1 expression in mouse PMs from WT or CKO mice. Data shown are means ± SD [n 5 6 in (A), and n 5 5 in (B)]. All experiments were repeated three times. **p < 0.01, ***p < 0.001, by Student t test.
Trim33 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trim33+gfp/GFP-TRIM33+(Plasmid+%2365398)/pm34497149-65-0-10
Average 90 stars, based on 1 article reviews
trim33 plasmid - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


FIGURE 2. KAP1 inhibits RNA viralinduced IFN-b activation. (A) Luciferase analysis of IFN-b reporter activation in HEK293T cells trans- fected with IFN-b reporter plasmid with empty control, TRIM28 (KAP1), TRIM24, TRIM33, or TRIM66 expression plasmid and then infected with SeV for 12 h. (B) Luciferase analysis of IFN-b reporter activation in HEK293T cells transfected with IFN-b reporter plasmid, together with empty control plasmid or KAP1 expression plasmids, and then infected with VSV for 12 h. (C) Western blot analysis of KAP1 expression in mouse PMs from WT or CKO mice. Data shown are means ± SD [n 5 6 in (A), and n 5 5 in (B)]. All experiments were repeated three times. **p < 0.01, ***p < 0.001, by Student t test.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: KAP1-Mediated Epigenetic Suppression in Anti-RNA Viral Responses by Direct Targeting RIG-I and MDA5.

doi: 10.4049/jimmunol.2100342

Figure Lengend Snippet: FIGURE 2. KAP1 inhibits RNA viralinduced IFN-b activation. (A) Luciferase analysis of IFN-b reporter activation in HEK293T cells trans- fected with IFN-b reporter plasmid with empty control, TRIM28 (KAP1), TRIM24, TRIM33, or TRIM66 expression plasmid and then infected with SeV for 12 h. (B) Luciferase analysis of IFN-b reporter activation in HEK293T cells transfected with IFN-b reporter plasmid, together with empty control plasmid or KAP1 expression plasmids, and then infected with VSV for 12 h. (C) Western blot analysis of KAP1 expression in mouse PMs from WT or CKO mice. Data shown are means ± SD [n 5 6 in (A), and n 5 5 in (B)]. All experiments were repeated three times. **p < 0.01, ***p < 0.001, by Student t test.

Article Snippet: TRIM33 plasmid (65398) and TRIM66 plasmid (65400) were purchased from Addgene and subcloned into the V5 tag to generate V5-TRIM33 and V5-TRIM66.

Techniques: Activation Assay, Luciferase, Plasmid Preparation, Control, Expressing, Infection, Transfection, Western Blot